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Human Papillomavirus (HPV) infection of the female genital tract has only recently been recognized as a widespread sexually transmitted disease that is associated with venereal warts and cervical neoplasia. The purpose of this study is to identify the relationship of HPV and CIN and invasive cervical cancer by using Southern blot hybridization technique and ViraPap HPV DNA detection kit. Using the southern blot hybridization techinque, tissue from 41 cases of invasive carcinoma of the uterine cervix, HPV DNA was detected in 37 of 42 cases (78.7%) under low stringency condition. On this positive group, high stage of the diseases revealed more high positive rate for HPV DNA than low stage of the diseases. HPV type 6/11, 16, 18, 52 and mixed infection were all detected in these sections, where as HPV type 16(70.3%) and 18(29.7%) were demonstrated distincitvely at a high rate than other HPV types among invasive carcinomas. A total of 71 cases of cervical tissues; 15 cases of cervical intraepithelial neoplasia (CIN), 50 cases of invasive cervical ancer, 6 cases of recurrent cervical cancer were analyzed by ViraPap HPV DNA detection kit. The positive rates for HPV type were 33.8% in which CIN, invasive cervical cancer and recurrent cervical cancer 13.3%, 42% and 16.7% respectively. In invasive cervical cancer, it was significantly higher than that of CIN group. According to the histological cell type, the positive rates for HPV for the squamous cell carcinoma and adenocarcinoma were 37.1% and 33.3% respectively, there was no significant difference in positive rate for HPV DNA. This study demonstrated that, the positive rates for HPV by using ViraPap HPV DNA detection kit was lower than using southern blot hybridization but ViraPap HPV DNA detection kit provides more convinent and rapid results. So that, careful medical history, physical examinations, Pap smear and ViraPap may be proride more rapid & accurate diagnosis of HPV infection.
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